产品简介：

    pCMV-Blank是上海华潍自行研发的用于在哺乳动物细胞中表达目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达。质粒为卡那霉素抗性。转染细胞后，可使用G418筛选稳定表达目的蛋白的细胞株。
    pCMV-Blank质粒的主要信息如下：
    Feature Nucleotide                                             Position
    CMV promoter                                                   1–602
    T3 promoter and T3 primer binding site                         620–639
    multiple cloning site                                          680–753
    T7 promoter and T7 primer binding site                         796–817
    SV40 polyA signal                                              829–1212
    f1 origin of ss-DNA replication                                1350–1654
    bla promoter                                                   1679–1803
    SV40 promoter                                                  1823–2161
    neomycin/kanamycin resistance ORF                              2196–2987
    HSV-thymidine kinase (TK) polyA signal                         2988–3446
    pUC origin                                                     3575–4242
        pCMV-Blank的多克隆位点的详细图谱如下：
                                       XmaI                 PstI
        SacI                           SmaI    BamHI  HindIII
    651 GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCAAGCTTCT
        CTCGAGGTGG CGCCACCGCC GGCGAGATCG GGCCCGCCTA GGTTCGAAGA
            EcoRI  EcoRV SalI  BglII  XhoI  XbaI         ApaI
    701 GCAGGAATTC GATATCGTCG ACAGATCTCT CGAGTCTAGA ACTAGTGGGC
        CGTCCTTAAG CTATAGCAGC TGTCTAGAGA GCTCAGATCT TGATCACCCG
          KpnI                KpnI
    751 CCGGTACCTT AATTAATTAA GGTACCAGGT AAGTGTACCC AATTCGCCCT
        GGCCATGGAA TTAATTAATT CCATGGTCCA TTCACATGGG TTAAGCGGGA 
    pCMV-Blank中没有的酶切位点(Restriction enzymes that do not cut pCMV-Blank)包括：
    Afl II    Age I    Ahd I       Asc I     Bbs I    Bbv II      Blp I
    Bsg I     BsiW I   BsmB I      BspM II   BsrG I   BssH II     Bst1107 I
    BstE II   Ear I    Eco47 III   Eco72 I   EcoN I   Esp I       Fse I
    Nru I     PflM I   Pme I       Pml I     PpuM I   Psp1406 I   Sap I
    Sca I     Spl I
    pCMV-Blank中的单酶切位点(Restriction enzymes that cut pCMV-Blank once)包括：

    pCMV-Blank质粒中对于插入片断进行测序时，推荐使用的正向测序引物T3和反向测序引物T7的序列
如下：
    T3 primer(620-639): 5′AATTAACCCTCACTAAAGGG 3′
    T7 primer(796-817): 5′GTAATACGACTCACTATAGGGC 3′
    pCMV-Blank的全序列信息： PHWE4379 pCMV-Blank-seq.txt